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目的探索APETX2对氯化锂-匹鲁卡品诱导痫性发作大鼠的行为学影响及可能的机制。方法成年雄性SPF级SD大鼠18只,侧脑室置管后随机分为:癫痫组(9只)、APETx2组(9只),癫痫造模后观察2组癫痫大发作潜伏期及发作强度;APETx2处理原代培养海马神经元,动态观察其对钙成像的影响。结果 APETx2组的SD大鼠癫痫潜伏期延长,大发作程度减轻;APETx2处理原代培养海马神经元钙内流下降。结论 APETx2可抑制氯化锂-匹鲁卡品诱导SD大鼠痫性发作,减少酸诱导海马神经元钙离子浓度增加可能为机制之一。
Objective To explore the effect of APETX2 on the behavior of rats induced by lithium chloride-pilocarpine-induced seizures and its possible mechanism. Methods Eighteen adult male Sprague-Dawley (SD) SD rats were randomly divided into epilepsy group (n = 9) and APETx2 group (n = 9). The latency and severity of epileptic seizures were observed after epilepsy model was established. APETx2 Treatment of primary cultured hippocampal neurons, dynamic observation of its impact on calcium imaging. Results The latency of epilepsy in APETx2 group was prolonged and the severity of major episode was alleviated. The calcium influx of primary cultured hippocampal neurons decreased in APETx2 group. Conclusions APETx2 may be one of the mechanisms by which lithium chloride-pilocarpine can induce epileptic seizures in SD rats and decrease the acid-induced increase of calcium concentration in hippocampal neurons.