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以叶片、叶柄或叶茎为外植体,在培养基MS+6-BA 1～2mg/L+NAA 0.1mg/L+2,4-D 0.1～0.2mg/L上均可诱导愈伤组织的产生,其中以MS+6-BA1.0 mg/L+NAA0.1mg/L+2,4-D 0.1mg/L效果最好,诱导率可达87%以上;其最佳的分化培养基MS+6-BA 1.5mg/L+NAA 0.2mg/L上,分化率可以达到90%以上,大哥大红掌最佳的增殖培养MS+6-BA 2mg/L+NAA0.2mg/L,培养30d芽的增殖系数达到3.45,降低6-BA浓度或提高NAA浓度有利于培养壮苗;1/2MS+NAA 0.1mg/L+1g/L活性炭的培养基诱导植株生根效果最好,生根率86%,平均每株苗生根3条;生根试管苗移栽于泥炭土:珍珠岩=5∶1的基质,成活率约86%。
The leaves, petioles or leaf stems were used as explants to induce callus in medium MS + 6-BA 1 ~ 2mg / L + NAA 0.1mg / L + 2,4-D 0.1 ~ 0.2mg / L The best induction rate could reach more than 87% with MS + 6-BA 1.0 mg / L + NAA 0.1 mg / L + 2,4-D 0.1 mg / L. The best differentiation medium The differentiation rate could reach more than 90% for MS + 6-BA 1.5mg / L + NAA 0.2mg / L, and the best multiplication culture of Anthurium andraeanum was MS + 6-BA 2mg / L + NAA0.2mg / The multiplication coefficient of buds reached 3.45. The reduction of 6-BA concentration or NAA concentration was conducive to the cultivation of strong seedlings. The rooting rate was 86% in medium supplemented with 1 / 2MS + NAA 0.1 mg / L and 1 g / L activated carbon, , The average rooting of each plant 3; rooting test tube seedlings transplanted in peat soil: perlite = 5: 1 matrix, the survival rate of about 86%.