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目的研究川芎嗪对失神经骨骼肌萎缩中FoXO3a、MAFbx及MuRF1表达的影响。方法 8周龄雌性SD大鼠54只,体重(200±10)g。随机分为3组,即正常对照组(A组,n=6)、失神经对照组(B组,n=24)、干预组(C组,n=24)。B、C组实验动物制备右下肢失神经腓肠肌动物模型,C组于模型制备后每日腹腔注射川芎嗪注射液80 mg/(kg.d),B组每日腹腔注射等剂量生理盐水;A组实验动物不作任何处理。取A组实验动物以及造模后2、7、14、28 d B、C组实验动物各6只,处死取其双侧腓肠肌称湿重,计算腓肠肌肌湿重比;另取右侧肌组织采用RT-PCR及Western blot检测各时间点FoXO3a、MAFbx、MuRF1 mRNA和蛋白表达水平。结果与A组比较,随着大鼠失神经时间延长,B、C组腓肠肌肌湿重比逐渐下降,差异均有统计学意义(P<0.05);造模后7、14、28 d,C组腓肠肌肌湿重比高于B组,且差异有统计学意义(P<0.05)。B、C组造模后7、14、28 d,FoXO3a、MAFbx、MuRF1 mRNA和蛋白表达水平均较A组高,C组各时间点均较B组降低,差异均有统计学意义(P<0.05)。结论川芎嗪可能通过降低FoXO3a、MAFbx以及MuRF1 mRNA和蛋白表达水平进而延缓失神经骨骼肌萎缩。
Objective To investigate the effects of ligustrazine on the expression of FoXO3a, MAFbx and MuRF1 in denervated skeletal muscle atrophy. Methods Fifty-four female Sprague-Dawley rats aged 8 weeks, weighing 200 ± 10 g. The rats were randomly divided into 3 groups: control group (n = 6), control group (n = 24) and intervention group (n = 24). Rats in group B and group C were given gastrocnemius animal model of denervated right lower extremity. Group C was given intraperitoneal injection of ligustrazine 80 mg / (kg.d) every day after the model was prepared. Group B was injected intraperitoneally with equal doses of normal saline. Group experimental animals without any treatment. A group of experimental animals and 2, 7, 14, 28 d after the modeling, C group 6 experimental animals, sacrificed bilateral gastrocnemius called wet weight, calculated gastrocnemius muscle wet weight ratio; the other right muscle tissue The expression of FoXO3a, MAFbx, MuRF1 mRNA and protein were detected by RT-PCR and Western blot. Results Compared with group A, with the prolongation of denervation in rats, the wet weight ratio of gastrocnemius muscle in groups B and C decreased gradually (P <0.05) The gastrocnemius muscle wet weight ratio was higher in group B than in group B, and the difference was statistically significant (P <0.05). The mRNA and protein expressions of FoXO3a, MAFbx and MuRF1 in group B and C were higher than those in group A at 7, 14 and 28 days after modeling, and were lower in group C than those in group B at each time point (P < 0.05). Conclusion Tetramethylpyrazine may delay denervated skeletal muscle atrophy by decreasing the expression of FoXO3a, MAFbx and MuRF1 mRNA and protein.