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目的观察吸烟对体内一氧化氮（ＮＯ）含量变化的影响及了解它们之间的关系。方法选择北京地区健康６０名男性不吸烟者（不吸烟组）及１９２名男性吸烟者，按其每日吸烟支数分为１～１０支组、１１～２０支组、２１～３０支组、＞３０支组。用硫代巴比妥酸法测定吸烟者尿液中尼古丁代谢产物柯替宁（ｃｏｔｉｎｉｎｓ）作为吸烟量的客观指标，用分光比色法测定尿中ＮＯ含量，来反映体内ＮＯ水平。结果吸烟者各组尿液中柯替宁含量分别为６．６±１．６μｍｏｌ／Ｌ、２５±６μｍｏｌ／Ｌ、３７±９ｍｏｌ／Ｌ、５４±８μｍｏｌ／Ｌ。不吸烟者尿液中ＮＯ含量为４．４±１．０μｍｏｌ／Ｌ，吸烟者各组尿液中ＮＯ含量分别为３．０４±０．０７μｍｏｌ／Ｌ、２．７５±０．０５μｍｏｌ／Ｌ、２．１８±０．０１μｍｏｌ／Ｌ、１．６０±０．０１μｍｏｌ／Ｌ。吸烟者尿液中柯替宁含量各组间依次分别为ｔ＝４．２８９４，Ｐ＜０．０１；ｔ＝２．７４４６、Ｐ＜０．０１；ｔ＝２．７６６７、Ｐ＜０．０１。吸烟者各组尿液中ＮＯ含量与不吸烟者组比较分别为ｔ＝２．７１１８、Ｐ＜０．０１；ｔ＝３．１８２６、Ｐ＜０．０１；ｔ＝３．２１００、Ｐ＜０．０１；ｔ＝４．６６００、Ｐ＜０．０１。尿液中ＮＯ?
Objective To observe the effects of smoking on the changes of nitric oxide (NO) content in the body and to understand the relationship between them. Methods Sixty male non-smokers (non-smokers) and 192 male smokers were enrolled in this study. They were divided into 1 to 10 groups, 11 to 20 groups and 21 to 30 groups according to the daily number of smokers. > 30 groups. Nicotine metabolite cotinins in urine of smokers was determined as an objective index of smoking by thiobarbituric acid method. The content of NO was measured by spectrophotometry to reflect the level of NO in the body. Results The urinary cotinine levels in smokers were 6.6 ± 1.6μmol / L, 25 ± 6μmol / L, 37 ± 9mol / L and 54 ± 8μmol / L, respectively. The content of NO in the urine of non-smokers was 4.4 ± 1.0μmol / L, and the NO in urine of smokers was 3.04 ± 0.07μmol / L and 2.75 ± 0.05μmol / L, 2.18 ± 0.01μmol / L, 1.60 ± 0.01μmol / L. The levels of cotinine in urine of smokers were respectively t = 4.2894, P <0.01; t = 2.7446, P <0.01; t = 2.7667, P <0.01 . The levels of NO in the urine of the smokers were significantly higher than those in the non-smokers, respectively (t = 2.7118, P <0.01; t = 3.1826, P <0.01; t = 3.2100, P < .01; t = 4.6600, P <0.01. NO in urine?