论文部分内容阅读
目的 :克隆表皮生长因子受体跨膜区 ( Transm em brane,TM )编码序列 ,为构建跨膜型超抗原和细胞因子的表达载体奠定基础。方法 :以表达 c- erb- B2癌基因的中国卵巢癌细胞系 HO- 8910细胞的 RN A逆转录产物( c DNA)为模板 ,用两条针对 c- erb- B2全长基因序列中编码 TM区序列特异性的引物 ,采用 RT- PCR方法克隆 T M区片段并测序。结果 :实验克隆的 TM编码序列 ,经测序证实与 Genbank中接受号为 M11730的 TM区序列完全一致 ;与接受号为 X0 336 3的 TM区序列存在 G→ A的多态性。结论 :成功地克隆了 TM编码序列 ,并证实源于中国的卵巢癌细胞系 H O- 8910中所包含的表皮生长因子受体基因中编码的 TM区序列存在多态性。
OBJECTIVE: To clone the coding sequence of Transmembrane (TM) transmembrane region and lay foundation for the construction of transmembrane superantigen and cytokine expression vector. Methods: RN A reverse transcription product (c DNA) of Chinese ovarian cancer cell line HO-8910 expressing c-erb-B2 oncogene was used as a template and two cDNAs coding for TM Region-specific primers were used to clone the TM region fragments by RT-PCR and sequenced. Results: The TM sequence of the experiment clone was completely consistent with the TM sequence of M11730 in Genbank and the G → A polymorphism of TM sequence of X0 336 3. CONCLUSION: The TM coding sequence was successfully cloned and confirmed that there is a polymorphism in the TM region sequences encoded by epidermal growth factor receptor genes contained in Chinese ovarian cancer cell line H O-8910.