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目的:比较小儿急性上呼吸道感染伴哮喘患者超敏C反应蛋白不同检验方法的灵敏度。方法:选取2012年10月—2013年10月期间收治的小儿急性上呼吸道感染伴哮喘患者120例,分别采用免疫透射比浊法和乳胶凝集比浊法检测患者超敏C反应蛋白水平值;比较2种检测方法的检测结果,并以飞测免疫荧光法检测结果为金标准分析检测结果的准确性和灵敏度。结果:应用免疫透射比浊法和乳胶凝集比浊法检测超敏C反应蛋白水平值,其结果示分别为(11.21±3.64)mg/L和(15.69±5.29)mg/L,两组患者的检测结果经比较其差异有统计学意义(t=3.15,P<0.05);用免疫透射比浊法检测结果的灵敏度、特异度、准确性、一致率和KAPPA值低于乳胶凝集比浊法的检测结果,两组资料经比较其差异有统计学意义(P<0.05)。结论:乳胶凝集比浊法的准确性和灵敏度均高于免疫透射比浊法。
OBJECTIVE: To compare the sensitivity of different test methods for Hs-CRP in children with acute upper respiratory infection and asthma. Methods: 120 children with acute upper respiratory tract infection and asthma admitted from October 2012 to October 2013 were selected. The levels of hypersensitivity C-reactive protein were detected by immunoturbidimetry and latex agglutination nephelometry respectively. 2 kinds of test methods, and the results of fly-based immunofluorescence test for the gold standard analysis of the accuracy and sensitivity of the test results. Results: The levels of hypersensitivity C-reactive protein were detected by immunoturbidimetry and latex agglutination nephelometry. The results showed that the levels of Hs-CRP were (11.21 ± 3.64) mg / L and (15.69 ± 5.29) mg / L respectively The sensitivity, specificity, accuracy, concordance rate and KAPPA of immunostaining turbidimetric assay were lower than those of latex agglutination turbidimetric assay (t = 3.15, P <0.05) Test results, the two groups of data by comparison the difference was statistically significant (P <0.05). Conclusion: The accuracy and sensitivity of latex agglutination turbidimetric method are higher than immunoturbidimetry.