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目的 研究抗运动神经元抗体在运动神经元病发病机制中的作用。方法应用抗运动神经元抗体作用于体外培养的大鼠神经元。结果 观察到抗运动神经元抗体对体外培养的大鼠神经元具有明显的细胞毒作用。将anti-SMN(1:50)加入培养的细胞中,24h后有31%～41%神经元死亡,48h后有50%～67%神经元死亡,72h后有90%以上神经元死亡。该细胞毒作用不依赖补体参与,强度与抗体浓度呈正相关。AchE组化染色发现anti-SMN对胆碱能神经元有选择性损伤作用,随暴露于抗体时间延长,AchE阳性神经元明显减少。CaBP免疫组化结果显示,CaBP阳性细胞在加入抗体48h达高峰,此后迅速下降,大部分阳性细胞在72h后死亡。结论 antiSMN抗体对胆碱能神经元有选择性细胞毒性作用,其机制可能与神经元内Ca~(2+)浓度的改变有关。
Objective To study the role of anti-motor neuron antibodies in the pathogenesis of motor neuron disease. Methods Anti-motor neuron antibodies were used to effect the in vitro cultured rat neurons. As a result, anti-motor neuron antibodies were found to have significant cytotoxic effects on rat neurons cultured in vitro. After anti-SMN (1:50) was added to the cultured cells, 31% ~ 41% neurons died after 24h, 50% ~ 67% neurons died after 48h, and more than 90% neurons died after 72h. The cytotoxic effect does not depend on complement participation, the intensity and antibody concentration was positively correlated. AchE staining showed anti-SMN selective cholinergic neurons injury, with prolonged exposure to antibodies, AchE positive neurons decreased significantly. CaBP immunohistochemistry results showed that CaBP positive cells peaked at 48h after antibody addition, then decreased rapidly, and most of the positive cells died after 72h. Conclusions antiSMN antibody has a selective cytotoxic effect on cholinergic neurons, which may be related to the change of intracellular Ca2 + concentration in neurons.