目的构建凝血栓蛋白-1Ⅰ型重复序列真核表达载体。方法采用RT-PCR技术,从人胎儿肺组织中扩增凝血栓蛋白-1Ⅰ型重复序列基因,通过连接反应构建重组克隆载体和重组表达载体,转化感受态大肠杆菌DH5α,筛选阳性克隆,双酶切鉴定及测序。结果获得了预期的扩增产物———凝血栓蛋白-1Ⅰ型重复序列基因,测序结果经GenBank分析,一致性为99%。结论成功构建了PcDNA3.1+/TSP-1Ⅰ型重复序列真核表达载体。 Objective To construct thrombospondin - Ⅰ Ⅰ repeat sequence eukaryotic expression vector. Methods The thrombospondin-1 typeⅠsequence was amplified from human fetal lung tissue by RT-PCR. The recombinant cloning vector and recombinant expression vector were constructed by ligation and transformed into competent E. coli DH5α. The positive clones were screened, Cut identification and sequencing. Results The expected amplified product, thrombospondin-1 type I repeats, was obtained. The sequencing results were analyzed by GenBank and the consistency was 99%. Conclusion The eukaryotic expression vector pcDNA3.1 + / TSP-1 Ⅰ was successfully constructed.