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目的探讨二甲基甲酰胺(DMF)致人肝细胞DNA的损伤情况及不同剂量VitC的干预作用。方法取正常成人肝细胞DMF染毒,DMF剂量为1.56、6.25、25、100 mmol/L、同时设阴性组与H2O2阳性组;100mmol/L DMF染毒后VitC的干预剂量为0.025、0.05、0.10、0.25mmol/L,应用单细胞凝胶电泳技术观察肝细胞DNA的损伤。结果同一处理时间组内各DMF染毒组与阴性对照组间MTL值与MTM值差异均有统计学意义(P<0.05)。不同剂量DMF处理肝细胞后,随着染毒剂量增加MTL值亦增加,最大MTL值出现于100mmol/LDMF组。各染毒组MTL值与MTM值与阴性对照组比较,差异有统计学意义(P<0.05)。结论高浓度DMF能引起肝细胞DNA损伤,小剂量VitC对DMF致肝细胞DNA损伤具有明显的拮抗作用。
Objective To investigate the DNA damage induced by dimethylformamide (DMF) in human hepatocytes and the intervention of different doses of VitC. Methods Normal adult hepatocytes were exposed to DMF for 1.56, 6.25, 25 and 100 mmol / L DMF, respectively. At the same time, negative group and H2O2-positive group were treated with 100 mg / L DMF. The intervention dose of VitC was 0.025,0.05,0.10 , 0.25mmol / L, single cell gel electrophoresis was used to observe DNA damage in hepatocytes. Results The differences of MTL and MTM between DMF-treated group and negative control group in the same treatment time group were statistically significant (P <0.05). After treatment with different doses of DMF, the MTL increased with the dose of MTT. The maximum MTL appeared in 100 mmol / L LDF group. The MTL value and MTM value in each exposure group were significantly different from those in the negative control group (P <0.05). Conclusion High concentration of DMF can cause DNA damage in hepatocytes, and low dose of VitC can antagonize DNA damage induced by DMF in liver cells.