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应用一步RNA提取法,改进经典cDNA文库构建程序,并用PCR技术鉴定cDNA文库的有效容量和插入cDNA的大小。从200mg小鼠小脑粒细胞中建立起容量为7×10~6个重组子的cDNA文库,为进一步研究小鼠缺陷株WEVER型的粒细胞丢失原因和基因表达缺陷提供正常小鼠粒细胞的cDNA文库。
A one-step RNA extraction method was used to improve the classical cDNA library construction program. The PCR library was used to identify the effective capacity of the cDNA library and the size of the inserted cDNA. A cDNA library of 7 × 10 ~ 6 recombinants was constructed from 200mg mouse cerebellar granulocytes. The cDNA of normal mouse granulocytes was provided to further investigate the causes of granulocyte loss and gene expression defects in WEVER mice library.