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目的 研究利拉鲁肽对高脂喂养肥胖大鼠胰腺中核转录因子κB(NF-κB)和蛋白酪氨酸磷酸酶1B(PTP-1B)表达的影响.方法 用高脂饮食诱导建立大鼠肥胖模型,并随机分组对照组和实验组,每组7只;另取7只正常大鼠作为空白组.实验组予以皮下注射利拉鲁肽注射液0.6 mg·kg-1·d-1,对照组和空白组均予以皮下注射同体积0.9％ NaCl约2 mL.3组大鼠均干预2周.比较3组大鼠胰腺中NF-κB活性和PTP-1B蛋白表达量.结果 干预2周后,实验组、对照组和空白组的NF-κB积分灰度值分别为69.2,150.5和70.4,PTP-1B蛋白表达量分别为(1.53±0.34),(1.79±0.12)和(1.32±0.34),实验组和空白组的上述指标比较,差异均无统计学意义(均P＞0.05),对照组的上述指标与实验组、空白组比较,差异均有统计学意义(均P＜0.05).结论 利拉鲁肽注射液可阻断高脂饮食对胰腺组织NF-κB的活化作用,同时可降低PTP-1B蛋白的表达.“,”Objective To observe the impact of liraglutide on expression of nuclear factor-kappa B (NF-κB) and protein tyrosine phosphatase -1B (PTP-1 B) in pancreas of the obese rats induced by high fat diet.Methods Obese rats model induced by high-fat-diet were randomly divided into control group (n =7 cases) and test group (n =7 cases).Another 7 normal rats were assigned to blank group.Mter modeling,test group received subcutaneous injection of liraglutide 0.6 mg · kg-1 · d-1 for 2 weeks.Control and blank groups took 2 mL subcutaneous injection of 0.9％ NaC1.We observed the expression and activity of NF-κB and PTP-1B in pancreas in three groups.Results After 2 weeks exposure,the activity of NF-κB in test,control and blank groups were 69.2,150.5 and 70.4,the expression of PTP-1B in test,control and blank groups were (1.53 ± 0.34),(1.79 ± 0.12) and (1.32 ± 0.34).The differences were statistically significant between blank group and test or blank groups (all P ＜ 0.05),and the differences were not significant between test group and blank group (all P ＞ 0.05).Conclusion Liraglutide injection can block the activation of NF-κB induced by high fat diet in pancreatic tissue thus reducing the expression of PTP-1 B,which implied that liraglutide might has been closely involved in the signal transduction pathway of NF-κB.