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从发病疫区栉孔扇贝(Chlamysfarreri)组织中分离出病毒和立克次体(Rickettsiaorganism,RO),对健康栉孔扇贝进行人工感染。结果显示,病毒注射组死亡率为75%,病毒浸浴组死亡率为68.7%;RO注射组死亡率仅为18.7%;灭活RO注射组死亡率为31%;灭活病毒注射和空白对照组死亡率皆为12.5%;病毒注射、浸浴组与灭活病毒注射组、空白对照组死亡率有显著差异。而RO注射组与灭活RO注射组、空白对照组死亡率没有显著差异。电镜复检结果显示,发病扇贝的外套膜、鳃、消化腺组织中分布有大量病毒粒子,该病毒粒子的形态特征、病理学特征与自然海区发病扇贝中的病毒粒子特征完全一致。人工感染实验结果证明,栉孔扇贝急性病毒性坏死病毒(A cuteVirusNecrobioticVirus,AVNV)是栉孔扇贝大规模死亡的直接病原。
Virus and Rickettsia organism (RO) were isolated from Chlamys farreri, and artificial infection of healthy scallop was conducted. The results showed that the death rate of the virus injection group was 75% and that of the virus immersion group was 68.7%; the mortality rate of RO injection group was only 18.7%; the mortality rate of inactivated RO injection group was 31%; inactivated virus injection and blank control Group mortality rate was 12.5%; virus injection, immersion group and inactivated virus injection group, the blank control group mortality were significantly different. The RO injection group and inactivated RO injection group, the control group mortality no significant difference. Electron microscopy results showed that a large number of virus particles were distributed in the mantle, gill and digestive gland of the diseased scallops. The morphological and pathological features of the virions were completely consistent with those of the virulent scallops in the natural sea area. The result of artificial infection proved that A virusVirus necrosis virus (AVNV) is the direct pathogen of large-scale death of Chlamys farreri.