以邻氨基苯硫酚和苄基氯为原料反应得到2-苄硫基苯胺(1),再将2-苄硫基苯胺(1)和罗丹明B进行缩合得到新型罗丹明B衍生物R1,并用IR,HRMS-ESI,~1H NMR以及13C NMR对其结构进行了表征.进一步通过荧光光谱法研究了化合物R1在甲醇水溶液中对常见离子的识别特性.结果表明荧光探针R1能够对Fe~(3+)进行快速,灵敏的识别,并且受其他常见离子干扰较小.探针R1的荧光强度与Fe~(3+)浓度(1×10~(-6)~9×10~(-6) mol/L)呈较好的线性关系,并在PC-12活细胞中实现了Fe~(3+)的荧光成像. The reaction of 2-benzylthioaniline (1) with o-aminothiophenol and benzyl chloride as raw materials gave the new rhodamine B derivatives R1 and 2-benzylthioaniline (1) The structure of compound R1 was characterized by IR, HRMS-ESI, 1H NMR and 13C NMR. The recognition properties of compound R1 for common ions in methanol aqueous solution were further studied by fluorescence spectroscopy. (3+) for rapid and sensitive identification, and it is less disturbed by other common ions.The fluorescence intensity of probe R1 is similar to that of Fe 3+ concentration (1 × 10 -6 ~ 9 × 10 ~ (- 6) mol / L), and fluorescence imaging of Fe ~ (3+) in PC-12 cells was achieved.