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摘要目的确定由巨噬细胞集落刺激因子(M-CSF)和粒细胞-巨噬细胞集落刺激因子(GM-CSF)刺激产生的两类巨噬细胞免疫代谢表型之间的差异以及这种差异对动脉粥样硬化斑块~(18)F-FDG显像的影响。材料与方法本研究已获动物保护委员会批准。评价小鼠腹膜未被激活的巨噬细胞(MΦ_0)以及被M-CSF(MΦ_(M-CSF))和GM-CSF(MΦ_(GM-CSF))激活的巨噬细胞的2-脱氧葡萄糖摄取及其有氧氧化和无氧酵解代谢指标。细胞内葡萄糖通量通过同位素示踪的糖酵解及三羧
Abstract Objective To determine the difference between the immune and metabolic phenotypes of macrophages stimulated by macrophage colony stimulating factor (M-CSF) and granulocyte-macrophage colony stimulating factor (GM-CSF) and the difference On atherosclerotic plaque ~ (18) F-FDG imaging. Materials and Methods This study has been approved by Animal Protection Committee. Evaluation of 2-deoxyglucose uptake by macrophages not activated peritoneum (MΦ_0) and macrophages activated by M-CSF (MΦ_ (M-CSF)) and GM-CSF (MΦ_ (GM-CSF) Its aerobic and anaerobic glycolysis metabolism indicators. Intracellular glucose flux is traced by isotope tracing of glycolysis and tricarboxylic