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以榕树(Ficus microcorpa)为试材,研究了冬季低温条件下不同浓度赤霉素(GA3)(0、15、150mg/L)和硫酸铵((NH4)2SO4)(0、20、200g/L)处理15、30、45d对活性氧代谢酶系统的愈创木酚过氧化物酶(G-POD)活性、抗坏血酸过氧化物酶(ASA-POD)活性、过氧化氢酶(CAT)活性、超氧化物歧化酶(SOD)活性和非酶系统丙二醛(MDA)含量的影响。结果表明:经GA3和(NH4)2SO4处理后可保持相对较高的抗氧化酶(POD、CAT、SOD)活性,削弱MDA含量的积累。在低温胁迫下随着GA3浓度的增加,POD活性、CAT活性和SOD活性呈先升高后降低的趋势;随(NH4)2SO4浓度的增加,POD活性、CAT活性和SOD活性呈增高的趋势,而MDA含量下降。含200g/L(NH4)2SO4的各处理15d后G-POD活性均表现得极为明显,15mg/L GA3+200g/L(NH4)2SO4处理下,AsA-POD活性均达到最高,15mg/L GA3+200g/L(NH4)2SO4处理30d后SOD活性持续上升并达峰值,15mg/L GA3+20g/L(NH4)2SO4处理15d后达到差异极显著水平(P<0.01);受GA3和(NH4)2SO4处理后榕树叶片MDA含量逐渐下降,在处理15d时达到最低;且30d和45d处理后整体MDA含量较15d有所升高。
The effects of different concentrations of GA3 (0, 15, 150 mg / L) and ammonium sulfate ((NH4) 2SO4) (0, 20, 200 g / L ) For 15, 30 and 45 days on the activity of G-POD, ASA-POD, CAT, Effects of superoxide dismutase (SOD) activity and non - enzymatic malondialdehyde (MDA) content. The results showed that GA3 and (NH4) 2SO4 could maintain relatively high activities of antioxidant enzymes (POD, CAT, SOD) and weaken the accumulation of MDA. With the increase of (NH4) 2SO4 concentration, POD activity, CAT activity and SOD activity increased firstly and then decreased with the increasing of GA3 concentration under low temperature stress. The MDA content decreased. After treated with 200g / L (NH4) 2SO4 for 15 days, the activities of G-POD were extremely obvious. Under the treatment of 15mg / L GA3 + 200g / L (NH4) 2SO4, the activities of AsA- (P <0.01). The activity of SOD was increased and reached the peak value after treated with 200 mg / L GA3 + 20 g / L (NH4) 2SO4 for 30 d, ) 2SO4 treatment, the content of MDA in leaves of banyan decreased gradually and reached the lowest at the 15th day after treatment. The content of MDA in the leaves of banyan was higher than that at the 15th day after 30d and 45d treatment.