目的:探索云南省师宗县蠓虫携带虫媒病毒情况。方法:采用RT-PCR方法对2013年7月采集自云南省师宗县的74批3 705只蠓虫进行检测，然后对扩增条带进行克隆测序。采用DNAStar软件中MegAlign进行同源性分析，使用MEGAX软件ALIGN进行序列比对和基于Maximum Likelihood (ML)方法进行遗传进化分析。结果:在云南省采集74批蠓虫样品中3批标本(SZC33、SZC42和SZ625C8-2)扩增出600 bp左右大小电泳条带；经过对以上PCR产物直接进行测序，SZC33和SZ625C8-2样本获得西藏环状病毒序列，而SZC42样品测序失败；对SZC42样品PCR产物进行克隆，挑取5个克隆进行测序，5个克隆均获得一段长为589个核苷酸(nucleotide，nt)序列，经BLASTx比对发现这5个克隆的核苷酸序列均与美国蚊虫中发现的星状病毒样病毒(culex bastrovirus-like virus, CAVL)的非结构蛋白基因氨基酸同源性最高，遗传进化分析结果显示云南省蠓虫(SZC42)样本检测到的病毒基因序列与美国蚊虫中检测到的CAVL(NC_040647)、巴西污水中检测到的星状病毒(Astrovirus，AV)(MF042208)以及越南蝙蝠检测到的AV(NC_032426)遗传进化关系较近，但形成独立的进化分支。结论:云南省蠓虫中发现的SZC42病毒序列为星状病毒序列。“,”Objective:To explore arboviruses carried by midges in Shizong county, Yunnan province.Methods:A total of 74 batches of 3705 midges collected from Shizong county, Yunnan province in July 2013 were detected by RT-PCR method, and then the amplified bands were cloned and sequenced. MegAlign in DNAStar software was used for homology analysis, MEGAX software ALIGN for sequence alignment and genetic evolution analysis based on Maximum Likelihood (ML) method .Results:Among 74 batches of midge samples collected in Yunnan province, 600 bp electrophoresis bands were amplified in 3 batches: SZC33, SZC42 and SZ625C8-2, while no electrophoresis bands were amplified in the other 71 batches of midge samples. After cloning, we selected 5 clones for sequencing, among which the 5 clones of sample SZC42 obtained 589 nt sequences. BLASTx comparison showed that the 5 cloned sequences of SZC42 had the highest amino acid homology(69%) with the non-structural protein gene of Culex bastrovirus-like(CAVL) virus found in mosquitoes collected in the United States, which was designated as Midge bastrovirus-like virus (MAVL). The result of genetic evolution analysis showed that MAVL detected in midges formed a single evolutionary branch, and it’s homology of nucleotides and amino acids with other astrovirus(AV) was less than 71.4%. Further analysis revealed that MAVL had a close genetic evolution relationship with CAVL (NC_040647) detected in American mosquitoes, AV (MF042208) detected in Brazilian sewage and AV (NC_032426) detected from Vietnamese bats, and the homology was 61.4%-66.2% (nt) and 67.7%-71.4% (aa), while far genetic evolution and low homology of nucleotides and amino acids with other AV sirains.Conclusions:A new astrovirus (MAVL) was detected in midges collected from Shizong county, Yunnan province in 2013.