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目的:观察杞菊地黄汤对实验性视网膜变性大鼠的治疗作用。方法:将生后45 d的SD大鼠分为正常组、模型组和杞菊地黄汤组。中药组大鼠ig杞菊地黄汤8.3 g.kg-1(15 mL.kg-1),模型组ig等体积生理盐水。两组动物均于生后47 d开始ig,每天1次,直至动物处死。生后50 d以N-甲基-N-亚硝脲(N-methyl-N-nitrosourea,MNU)ip造成视网膜变性模型,预定时间行闪光视网膜电图(flash electroretinograms,FERG)检测,造模后2,5 d时分批处死动物,取右眼行病理切片,分别以TUNEL法检测视网膜外核层细胞的凋亡、HE染色检测视网膜外核层的厚度。结果:ig杞菊地黄汤8.3 g.kg-1组大鼠FERG消失时间较模型对照组明显延迟;造模后2 d,其外核层细胞凋亡指数(50.8±7.3)%低于同期模型组大鼠(81.1±9.7)%(P<0.01);造模后5 d,其视网膜外核层厚度(71±27)μm大于同期模型组大鼠(22±10)μm(P<0.01)。结论:杞菊地黄汤对MNU诱导的光感受器细胞凋亡有保护作用。
Objective: To observe the therapeutic effect of Qiju Dihuang Decoction on experimental retinal degeneration in rats. Methods: The SD rats of 45 days after birth were divided into normal group, model group and Qiju Dihuang Decoction group. Chinese medicine group ig Qi Ju Di Huang Tang 8.3 g.kg-1 (15 mL.kg-1), the model group ig equal volume of saline. Animals in both groups began ig 47 days after birth, once daily until the animals were sacrificed. The model of retinal degeneration was induced by N-methyl-N-nitrosourea (MNU) ip at 50 days after birth. The rats were examined by flash electroretinograms (FERG) Animals were sacrificed on day 2 and day 2, and pathological sections of the right eye were taken. TUNEL assay was used to detect the apoptosis of extranodal outer nuclear layer cells. HE staining was used to measure the thickness of the outer retinal layer. Results: Compared with the model control group, the disappearance time of FERG in 8.3 g.kg-1 group was significantly delayed and the apoptotic index of the outer nuclear layer was (50.8 ± 7.3)% lower than that of the model group (81 ± 9.7)% (P <0.01). At 5 days after modeling, the thickness of the outer retinal layer (71 ± 27 μm) was larger than that of the model group (22 ± 10 μm) (P <0.01). Conclusion: Qiju Dihuang Decoction can protect MNU-induced apoptosis of photoreceptor cells.