金纳米颗粒(GNPs),由于其具有良好的生物稳定性、独特的光电和物理化学特性,已被广泛用于纳米医学和神经科学.但是,不同形貌的GNPs对神经元的神经生理特性的影响仍不清楚.为解决此问题,合成了不同形状的GNPs(纳米球,纳米六面体和纳米花)以研究不同表面粗糙度的GNPs对小鼠海马CA1神经元电压依赖性钠通道和动作电位(AP)的影响.结果表明,GNPs抑制了电压门控钠电流(INa)的电流幅度,使INa激活和失活的电压依赖性激活和失活曲线向超级化方向偏移.GNPs还增加了神经元兴奋性并改变了动作电位的幅度、阈值、半波宽等参数变化.此外,在纳米花GNPs处理过的CA1神经元中观察到了强度最大的AP改变(六面体次之,球最小),这表明金纳米颗粒的神经毒性与表面粗糙度呈正相关.这些结果可能为GNPs的临床应用提供有价值的参考.“,”Gold nanoparticles (GNPs) have been extensively used in nanomedicine and neuroscience owing to their biological inertness, peculiar opto-electronic and physico-chemical features. However, the effect of GNPs shape on the neurophysiological properties of single neuron is still unclear. To tackle this issue, different shape GNPs (nanosphere, nanotriakisoctahedron and nanoflower) were synthesized to investigate the effect of GNPs on the voltage-dependent sodium channel and the action potential (AP) of hippocampal CA1 neurons in mice. The results indicated that GNPs inhibited the amplitudes of voltage-gated sodium current (INa) and led to a hyperpolarizing shift in the voltage-dependence curve of both activation and inactivation of INa. GNPs also increased neuronal excitability and altered some properties of AP. Moreover, most alterations in AP properties were observed in nanoflower GNPs treated CA1 neurons, suggesting that the neurotoxicity of gold nanoparticles is surface roughness-dependent. These results may provide a valuable direction in the clinical application of GNPs.