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目的:确定脐血中高水平的造血因子的来源。方法:应用抗粒单核细胞集落刺激因子(GMCSF)和抗粒细胞集落刺激因子(GCSF)的单克隆抗体对19例健康产妇足月分娩的胎盘组织的冰冻切片进行免疫组织化学染色。以耳鼻咽喉科摘取的鼻息肉组织做对照。结果:胎盘郎汉斯细胞及合体细胞呈现阳性反应,而阴性对照无反应。结论:胎盘滋养层细胞可能是产生脐血中GMCSF和GCSF的主要细胞
Purpose: To determine the origin of high levels of hematopoietic factors in umbilical cord blood. Methods: Immunohistochemistry was performed on frozen sections of 19 healthy pregnant women with full-term delivery of placental tissue using monoclonal antibodies against GM-CSF and G-CSF. dyeing. Nasopharyngeal tissue extracted from otolaryngology was used as a control. Results: The Langerhans cells and syncytial cells of the placenta showed a positive reaction, while the negative control did not respond. Conclusion: Placental trophoblast cells may be the main cells of cord blood GM-CSF and G-CSF