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目的:分析桃红四物汤抑制小鼠痛经有效部位的谱效关系,以阐明相关的物质基础。方法:采用己烯雌酚结合缩宫素致小鼠痛经模型,对桃红四物汤及其单味药石油醚部位进行筛选;并结合GC-MS法对其分析,运用数理统计方法将药理数据与气相色谱峰相对面积相关联。结果:桃红四物汤的石油醚部位有显著的调经止痛作用。桃红四物汤亲脂性化学成分主要为脂肪酸类成分。综合回归分析与相关分析结果表明:23号峰即亚油酸在延长扭体潜伏期和减少扭体次数方面起主要作用,其次24号峰(十八碳烯酸),45号峰(花生酸)和61号峰(二十七烷)对镇痛效果有一定的协同作用。结论:本方法结合药效筛选和化学分析,运用相应的数理统计,可以较客观的分析评价化学物质与效应之间的关系。
Objective: To analyze the effect of Taohong Siwu decoction on the effective site of mice dysmenorrhea to elucidate the related material basis. Methods: The mice model of dysmenorrhea induced by diethylstilbestrol and oxytocin was used to screen the Taohong Siwu Decoction and its single herbaceous petrolatum site. Combined with GC-MS analysis, the pharmacological data were compared with gas chromatography Peak relative area is associated. Results: peach red soup parts of the petroleum ether have significant menstrual pain relief role. The lipophilic chemical components of Taohong Siwu Tang are mainly fatty acid components. The results of regression analysis and correlation analysis showed that linoleic acid at peak 23 played a major role in prolonging the latency of writhing and reducing the number of writhing. Followed by peak 24 (octadecenoic acid), peak 45 (arachidic acid) And No. 61 peak (heptacosane) have a synergistic effect on the analgesic effect. Conclusion: This method combined with efficacy screening and chemical analysis, using the corresponding mathematical statistics, can be more objective evaluation of the relationship between chemical substances and the effect.