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目的报告一起宋内志贺菌暴发的病原学鉴定及其病原学特征。方法在流行病学调查、临床表现基础上,采集病人粪便,进行腹泻病多重PCR检测进行初步判定病原,同时进行实验室细菌分离培养和病原学鉴定,对分离得到的病原菌进行药物敏感性和毒力基因的检测。结果对15份粪便标本进行多重PCR方法进行检测,13份标本志贺菌显示为阳性。对30份病例的粪便进行细菌分离培养,分离得到宋内志贺菌6株。本次疫情中分离得到的志贺菌株,药物敏感试验结果显示对青霉素类及头孢类抗生素耐药,毒力基因的检测结果显示菌株携带有sen、ial、ipaH此3种毒力基因,不携带毒力基因set1。结论本次疫情为一起由宋内志贺菌引起的细菌性痢疾暴发流行。多重PCR检测可作为辅助检测手段,及时明确病原,为临床治疗和疫情应急处置提供积极参考。分离得到的志贺菌株对呈现多重耐药现象,且携带志贺菌主要毒力基因,致病力强。
Objective To report the etiological identification and etiological characteristics of Shigella sonnei in Song. Methods On the basis of epidemiological investigation and clinical manifestations, we collected the excrement of the patients and carried out the multiplex PCR detection of diarrhea to determine the pathogen. At the same time, we isolated and cultured the bacteria in the laboratory and identified the etiology. The drug sensitivity and toxicity Force gene testing. Results Fifteen stool specimens were tested by multiplex PCR and 13 specimens were positive for Shigella. Bacteria were isolated from 30 cases of stool samples and 6 strains of Shigella sonnei were isolated. The Shigella strain isolated from this outbreak showed drug resistance test to penicillins and cephalosporins. The results of virulence genes showed that the strain carried the three virulence genes, sen, ial and ipaH, without carrying Virulence gene set1. Conclusion The outbreak was caused by the outbreak of bacillary dysentery caused by Shigella sonnei. Multiplex PCR detection can be used as an auxiliary detection means to identify pathogens in time, providing a positive reference for clinical treatment and emergency treatment. Isolated Shiga strains showed multiple drug resistance, and carry Shigella major virulence genes, strong pathogenicity.