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目的:观察地黄多糖对SD大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)增殖及骨向分化的影响。方法:采用全骨髓贴壁法分离培养SD大鼠骨髓间充质干细胞;MTT法检测不同浓度地黄多糖在不同时间点(1、3、5、7、9,、11 d)对大鼠BMSCs增殖情况的影响;对硝基苯磷酸盐(pNPP)法检测不同浓度地黄多糖在不同时间点(3、7、14 d)对细胞上清中ALP活性的影响;茜素红染色法检测不同浓度地黄多糖对BMSCs矿化能力的影响。结果:地黄多糖可以促进BMSCs增殖,其最佳浓度为50μg/m L,P<0.05;同时可以促进BMSCs向成骨分化,其最佳浓度也为50μg/m L,P<0.05。结论:地黄多糖具有促BMSCs增殖及骨向分化的作用。
Objective: To observe the effects of Rehmannia glutinosa polysaccharide on the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs) of SD rats. METHODS: SD rat bone marrow mesenchymal stem cells were isolated and cultured by whole bone marrow adherent method. The proliferation of BMSCs was detected by MTT assay at different time points (1, 3, 5, 7, 9, 11 d) (PNPP) method was used to test the effects of different concentrations of Rhizoma Polygonatum polysaccharide on ALP activity in cell supernatant at different time points (3, 7, 14 days). Alizarin red staining was used to detect the effects of different concentrations of Rehmannia glutinosa Effect of polysaccharide on mineralization of BMSCs. Results: Rehmannia polysaccharide could promote the proliferation of BMSCs. The optimum concentration of Rehmannia glutinosa polysaccharide was 50μg / m L, P <0.05. At the same time, BMSCs could promote the osteogenic differentiation. The best concentration was 50μg / m L, P <0.05. Conclusion: Rehmannia polysaccharide can promote the proliferation and differentiation of BMSCs.