【摘 要】
：
Protein dephosphorylation mediated by protein phosphatases plays a major role in signal transduction of plant responses to environmental stresses.In this study,
【机 构】
：
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Robert Holley
论文部分内容阅读
Protein dephosphorylation mediated by protein phosphatases plays a major role in signal transduction of plant responses to environmental stresses.In this study,two putative protein phosphatases,PvPS2:1 and PvPS2:2 were identified and characterized in bean (Phaseolus vulgaris).The two PvPS2 members were found to be localized to the plasma membrane and the nucleus by transient expression of PvPS2:GFP in onion epidermal cells.Transcripts of the two PvPS2 genes were significantly increased by phosphate (Pi) starvation in the two bean genotypes,G19833 (a P-efficient genotype) and DOR364 (a P-inefficient genotype).However,G19833 exhibited higher PvPS2:1 expression levels than DOR364 in both leaves and roots during P1 starvation.Increased transcription of PvPS2:1 in response to Pi starvation was further verified through histochemical analysis of PvPS2:1 promoter fusion β-glucuronidase (GUS) in transgenic Arabidopsis plants.Analysis of PvPS2∶1 overexpression lines in bean hairy roots and Arabidopsis showed that PvS2:1 was involved in root growth and P accumulation.Furthermore,expression levels of two P(1) starvation responsive genes were upregulated and the APase activities were enhanced in the overexpressing PvPS2∶1 Arabidopsis lines.Taken together,our results strongly suggested that PvPS2∶1positively regulated plant responses to P1 starvation,and could be further targeted as a candidate gene to improve crop P efficiency.
其他文献
Brassinosteroids (BRs),a group of plant steroidal hormones,play critical roles in many aspects of plant growth and development.Previous studies showed that BRI1
Background Chronic obstructive pulmonary diseases (COPD) is an emerging population at risk for invasive infection of Aspergillus.Isolation of Aspergillus from l
Xylem sap primarily transports water and mineral nutrients such as nitrogen (N) from roots to shoots in vascular plants.However,it remains largely unknown how n
Four wheat (Triticum aestivum L.) cultivars 711,PBW343,3765 and WH542 were screened for studying variations in glycinebetaine (GB) content and plant dry mass un
Background The computed tomography (CT) findings of invasive pulmonary aspergillosis (IPA) are unclear in nonhematological patients.The present study was a retr
Silicon (Si) can enhance the resistance of plants to many abiotic stresses. To explore whether Si ameliorates Fe2+ toxicity,a hydroponic experiment was performe
研究了不同钙含量对铸态下医用Mg-6%Zn-x%Ca镁合金的显微组织和力学性能的影响。结果表明,随着钙含量的增加,合金的晶粒尺寸明显变小,显微硬度明显提高;能谱分析发现,钙主要
A technology to achieve stable and high ammonia nitrogen removal rates for corn distillery wastewater (ethanol fuel production) treatment has been designed.The
Nitrogen use efficiency (NUE) was very low in China and a loss of as much as 70％ of the applied nitrogen fertilizers was reported in high-yielding rice fields.In