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1987年9月～1988年2月我们采用聚丙酰胺凝胶电泳法检测78例人轮状病毒(HumanRota Virus,HRV)阳性病例。其中男50例,女29例。<1岁33例,1～3岁45例,4岁1例。均为本院门诊和病房的腹泻患儿。实验方法:1.核酸提取:取0.3ml粪便上清液加10％SDS30ul置56℃水浴30分钟,再加0.3ml TES饱和酚0.2ml氯仿-异戌醇(24:1),振摇2分钟,4,000rpm离心5分钟,上层水相即为所提RNA。2.电泳(核酸分离):电泳前先将样品置60℃水浴2分钟,再每份样品取
From September 1987 to February 1988 we detected 78 humanRota Virus (HRV) positive cases by polyacrylamide gel electrophoresis. There were 50 males and 29 females. <1 year old 33 cases, 1 ~ 3 years old 45 cases, 4 years old in 1 case. All of our clinics and wards had diarrhea children. Experimental methods: 1. Nucleic acid extraction: Take 0.3ml fecal supernatant plus 10% SDS30ul 56 ℃ water bath for 30 minutes, then add 0.3ml TES saturated phenol 0.2ml chloroform - isoalcohol (24: 1), shaken for 2 minutes , 4,000rpm centrifuge for 5 minutes, the upper aqueous phase is the RNA. 2. Electrophoresis (nucleic acid separation): electrophoresis before the sample was placed 60 ℃ water bath for 2 minutes, and then each sample taken