观察烟草特异亚硝胺ＮＮＫ在诱发人胎肺组织（ＨＦＬＴ）癌变中的启动作用。结果显示：（１）在０～５００μｇ／ｍｌ范围内，ＨＬＦＴ中８ＯＨｄＧ含量随ＮＮＫ剂量的增加而增高；（２）５００μｇ／ｍｌＮＮＫ处理组ＨＬＦＴＤＮＡ与Ｎｅｕ基因共转染后所得Ｇ４１８抗性细胞可在裸鼠体内形成纤维肉瘤，且细胞形态呈多角形，提示ＮＮＫ所致ＨＬＦＴＤＮＡ损伤使其获得了转化Ｒａｔ１细胞的潜能。说明ＮＮＫ在诱导ＨＬＦＴ癌变中发挥启动作用，并为研究肺癌早期分子标志提供了线索 Observed the start-up effect of tobacco-specific nitrosamine NNK in the induction of carcinogenesis of human fetal lung tissue (HFLT). The results showed that: (1) in the range of 0-500μg/ml, the content of 8-OH-dG in HLFT increased with the increase of the dose of NNK; (2) the anti-G418 protein was co-transfected with NN and HLFTDNA in NNK-treated group at 500μg/ml. Sexual cells can form fibrosarcoma in nude mice, and the cell morphology presents a polygonal shape, suggesting that NNK-induced HLFT DNA damage confers the potential to transform Rat1 cells. Explain that NNK plays a priming role in inducing HLFT carcinogenesis and provides clues for the early molecular markers of lung cancer