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目的探讨雌激素对大鼠门静脉高压性胃病的影响及其机制。方法40只SD大鼠被随机分为4组:P+E组接受门静脉主干结扎+雌激素;P组仅接受门静脉主干结扎;S+E组接受假手术+雌激素;S组仅接受假手术。所有大鼠维持相应处理14 d后处死,处死前1h予2 ml 99％乙醇灌胃。用激光多普勒仪检测胃黏膜血流量,计算胃黏膜损伤程度。用动态一氧化氮(NO)检测仪检测胃黏膜NO含量等。结果P+E组胃黏膜血流量(103±14)U显著高于其他3组(P<0．05)。胃黏膜损伤指数:P+E组(0．28±0．17)、P组(0．21±0．08)和S+E组(0．21±0．12)组均显著高于S组(0．11±0．06),P<0．05。P+E组的NO测量值(153±23)nmol／L显著高于P组(123±14) nmol／L、S+E组(116±18)nmol／L和S组(104±15)nmol／L,P<0．05。结论雌激素加重门静脉高压症大鼠胃黏膜的损伤,可能与其促进胃黏膜内NO的产生有关。
Objective To investigate the effect of estrogen on portal hypertensive gastropathy in rats and its mechanism. Methods 40 SD rats were randomly divided into 4 groups: P + E group received portal vein ligation + estrogen; P group received only portal vein ligation; S + E group received sham + estrogen; S group received only sham operation . All rats were sacrificed 14 days after the corresponding treatment and sacrificed 2 ml of 99% ethanol 1 h before sacrifice. Gastric mucosal blood flow was measured by laser Doppler and the degree of gastric mucosal damage was calculated. Using dynamic nitric oxide (NO) detector to detect gastric mucosal NO content. Results The gastric mucosal blood flow (103 ± 14) U in P + E group was significantly higher than that in the other three groups (P <0.05). Gastric mucosal injury index was significantly higher in the P + E group (0.28 ± 0.17), P group (0.21 ± 0.08) and S + E group (0.21 ± 0.12) than in the S Group (0.11 ± 0.06), P <0.05. NO measured in the P + E group was significantly higher (153 ± 23) nmol / L than in the P group (123 ± 14) nmol / L, in the S + E group (116 ± 18) nmol / L and in the S group nmol / L, P <0.05. Conclusion Estrogen may aggravate the damage of gastric mucosa in rats with portal hypertension, which may be related to the promotion of NO production in gastric mucosa.