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通过给切除部分肝脏大鼠注射鲨鱼肝再生因子,观察其对肝脏细胞再生的促进作用,为临床用药提供依据。按Higgins和Anderson方法对大鼠行肝切除术,分别尾静脉注射鲨鱼肝再生因子(SHRF)12,6,3 mg/kg,促肝细胞生长素(HGF 6 mg/kg),生理盐水(0.3 ml/100 g)术后10 d取肝脏称重及记录。并对各时相点大鼠取血和肝细胞培养。比较各组大鼠血清甲胎蛋白(AFP)和肝细胞中一氧化氮(NO)含量的变化。结果给药组与模型组肝脏重量比较有极显著差异,给药组和阳性药组血清中AFP及肝细胞中NO含量与模型组相比均呈升高趋势,因此SHRF在短期内对大鼠肝脏部分切除术后再生有明显的促进作用。
By injecting shark liver regeneration factor into part of the liver of rats and observing its promotion effect on liver cell regeneration, provide basis for clinical use. According to the method of Higgins and Anderson, rats were subjected to hepatectomy. Shark liver regeneration factor (SHRF) 12, 6, and 3 mg / kg, HGF 6 mg / kg and saline 0.3 ml / 100 g) The liver was weighed and recorded 10 days after operation. Blood samples were taken from rats at each time point and hepatocytes were cultured. The levels of serum alpha-fetoprotein (AFP) and nitric oxide (NO) in hepatocytes of each group were compared. Results Compared with the model group, the liver weight of the administration group and the model group had a very significant difference. The serum AFP level in the administration group and the positive drug group were higher than those in the model group. Therefore, Partial liver regeneration after resection has a significant role in promoting.