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目的:通过观察参芪补心丸对心肌缺血模型大鼠脂联素(APN)、脂蛋白相关磷脂酶A2(LP-PLA2)水平表达影响,探讨参芪补心丸治疗冠心病的作用机制和疗效。方法 :健康wistar大鼠60只,雌雄各半,用随机数字表分组法将它们分为6组:空白组、模型组、心通口服液组及参芪补心丸高、中、低剂量组,每组10只。利用酶联免疫吸附技术观察参芪补心丸对心肌缺血模型大鼠血清APN、LP-PLA2水平表达的影响。结果 :与空白组比较,模型对照组大鼠血清脂联素(APN)水平降低,而脂蛋白相关磷脂酶A2(LP-PLA2)水平升高有显著差异(P<0.05);与模型组比较,心通口服液组、参芪补心丸各剂量组大鼠血清脂联素(APN)水平均升高,而大鼠血清LP-PLA2均水平降低,有显著差异(P<0.05);与心通口服液组比较,参芪补心丸高、中剂量组脂联素(APN)水平升高,而脂蛋白相关磷脂酶A2(LP-PLA2)水平降低,有显著差异(P<0.05),低剂量组脂联素(APN)、脂蛋白相关磷脂酶A2(LP-PLA2)水平与心通组比较差异不明显(P>0.05)。结论:参芪补心丸能明显升高心肌缺血模型大鼠血清APN水平;参芪补心丸能明显降低心肌缺血模型大鼠血清LP-PLA2水平;参芪补心丸能减轻血管内皮炎性反应,抗血管内皮损伤,抗动脉粥样硬化。
Objective: To observe the effect of Shenqi Buxin Wan on the expression of adiponectin (APN) and lipoprotein-associated phospholipase A2 (LP-PLA2) in rats with myocardial ischemia and to explore the mechanism of action of Shenqi Buxinwan in treating coronary heart disease And efficacy. Methods: Sixty healthy wistar rats were divided into 6 groups according to the random number table method: blank group, model group, Xintong oral liquid group and Shenqi Buxinwan high, medium and low dose groups , Each group of 10. The effect of Shenqi Buxinwan on serum APN, LP-PLA2 expression in myocardial ischemia model rats was observed by enzyme-linked immunosorbent assay. Results: Compared with the blank group, serum adiponectin (APN) level and LP-PLA2 level increased significantly in the model control group (P <0.05). Compared with the model group , Xintong Oral Liquid Group and Shenqi Buxin Pill group all increased serum adiponectin (APN) level, while serum LP-PLA2 level in rats decreased significantly (P <0.05), and Compared with Xintong Oral Liquid group, the levels of APN in Shenqi Buxinwan high and middle dose groups increased, while the level of LP-PLA2 decreased (P <0.05) , APN and LP-PLA2 in low dose group were not significantly different from those in Xintong group (P> 0.05). Conclusion: Shenqi Buxin Pill can significantly increase serum APN level in rats with myocardial ischemia; Shenqi Buxin Pill can significantly reduce serum levels of LP-PLA2 in rats with myocardial ischemia; Shenqi Buxin Pill can reduce intravascular Dermatitis, anti-vascular endothelial injury, anti-atherosclerosis.