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研究了在奎宁存在下四碘荧光素与新洁而灭的缔合反应。在pH 7.9～8.6范围内新浩而灭与四碘荧光素、奎宁形成稳定的三元离子缔合物(λ_(max)545 nm)。用1,2-二氯乙烷萃取时e=5.8×10~4 1·mol~(-1)·cm~(-1)。测出缔合物组成为1∶1∶1。研究了测定微量新浩而灭的条件和方法。2×10_(-7)～4×10_(-6)M新洁而灭符合Lambert-Beer定律,多数胺与生物碱不干扰测定。比较了在奎宁存在下17种酸性染料与新洁而灭的萃取—分光光度体系,确定荧光素类显色剂的效果优于类似结构的磺酞类显色剂。
The association reaction of tetraiodo-fluorescein with neo-Jiemie was studied in the presence of quinine. In the range of pH 7.9 ~ 8.6, a new ternary ion association (λ max (545 nm)) with tetraiodofluorescein and quinine was formed. When extracted with 1,2 - dichloroethane, e = 5.8 × 10 ~ 4 1 · mol ~ (-1) · cm ~ (-1). The association was determined to be 1: 1: 1. The conditions and methods for determining the amount of micro-new ho disappeared. 2 × 10 _ (- 7) ~ 4 × 10 _ (- 6) M Neo Jie and kill comply with Lambert-Beer law, most of the amine and alkaloids do not interfere with the determination. 17 kinds of acid dyes in the presence of quinine were compared with the neo-extinction extraction-spectrophotometry system to determine the effect of fluorescein-based color-developing agent over the similar structure of sulf-phthalide-based color-developing agent.