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提出了具有高探测灵敏度的微腔光声探测技术。采用微腔耦合技术,将细胞内产生的光声信号,通过微通道传导到耦合腔,被麦克风接收,成功研制出微腔光声探测器。在相同的温度变化情况下,密闭腔内气压变化量与腔的体积成反比,腔的体积越小,气压变化量越大,探测器对压力的响应度就越高,对于微弱光声信号就越灵敏。因此,设计出的高灵敏度微腔光声探测器,能够探测到连续调制光激发出的微弱光声信号,重建出免标记的细胞水平的光声显微图像。从已获得的分辨率板、洋葱表皮细胞和血红细胞等样品的光声显微图像可以说明这个光声微腔探测器的灵敏度高,分辨率达到1.25μm。
A micro-cavity photoacoustic detection technology with high detection sensitivity is proposed. Using microcavity coupling technology, the photoacoustic signal generated in the cell is transmitted to the coupling cavity through the microchannel and received by the microphone, and a micro-cavity photoacoustic probe is successfully developed. Under the same temperature change, the pressure variation in the airtight chamber is inversely proportional to the volume of the chamber. The smaller the volume of the chamber is, the larger the pressure variation is and the higher the responsivity of the detector to pressure is. For a weak photoacoustic signal The more sensitive. Therefore, the designed high-sensitivity micro-cavity photoacoustic probe can detect weak photoacoustic signals excited by continuous modulated light and reconstruct photoacoustic microscopy images at unlabeled cells. Photoacoustic microscopy images of obtained resolution plates, onion epidermal cells and red blood cells demonstrate the high sensitivity of this photoacoustic micro-cavity detector with a resolution of 1.25 μm.