为了获取日本血吸虫特异的cDNA克隆，用日本血吸虫病流行区化疗后再感染人群血清筛选构建于表达载体λt11的日本血吸虫成虫cDNA文库，再以多聚酶链反应（PCR）技术鉴定免疫筛选的阳性cDNA克隆。结果对cDNA表达库中约8．6×104个噬菌斑进行了筛选，初筛时共挑出59个可能的阳性斑，经两次复筛后，有11个噬菌斑仍呈阳性反应。以阳性噬菌斑DNA为模板，经PCR均扩增出一定大小的片段，从564bp到740bp不等。 In order to obtain specific cDNA clones of Schistosoma japonicum, the cDNA library of adult Schistosoma japonicum cDNA library constructed with the expression vector λt11 was screened by serum of re-infected human in the area where schistosomiasis japonica was treated. The positive cDNA clones of immunoscreening were identified by polymerase chain reaction (PCR) . Results A total of 8.6 × 104 plaques were screened in the cDNA expression library. A total of 59 possible positive plaques were picked out during the primary screening. After two rounds of screening, 11 plaques were still positive . Positive plaque DNA was used as a template to amplify some fragments by PCR, ranging from 564bp to 740bp.