论文部分内容阅读
目的 :研究乙肝病毒 (HBV)DNA整合到精子染色体的特点。方法 :选 14例精子染色体样本 ( 5例健康对照 ,9例乙肝病人 ,包括 1例急性乙型肝炎 ,2例慢性活动性乙肝 ,4例慢性迁延性乙肝 ,2例无临床症状的乙型肝炎表面抗原携带者 ) ,用于仓鼠无透明带卵母细胞和人类精子的种间体外受精。用生物素标记的全长HBVDNA探针进行荧光原位杂交至精子染色体 ,以检测人类精子染色体特异性HBVDNA序列。结果 :1例慢性迁延性乙肝病人精子染色体中检测到HBVDNA特异性荧光信号。 4 2对精子染色体补体中 ,9对含特异性荧光信号 ,其中 1对出现 5个明显的FISH点 ,其余有 2～ 4个信号点。荧光信号强度有显著差异 ,信号位点分布似乎是随机的。结论 :HBV可以整合入人类精子染色体中 ,结果提示HBV可能垂直传播到下一代
Objective: To study the characteristics of integration of hepatitis B virus (HBV) DNA into sperm chromosomes. Methods: 14 cases of sperm chromosome samples (5 healthy controls, 9 hepatitis B patients, including 1 case of acute hepatitis B, 2 cases of chronic active hepatitis B, 4 cases of chronic persistent hepatitis B, 2 cases of clinical symptoms of hepatitis B Surface antigen carriers) for in vitro fertilization of hamster zona-free oocytes and human sperm. Fluorescence in situ hybridization to sperm chromosomes with biotin-labeled full-length HBVDNA probes to detect human sperm chromosome-specific HBVDNA sequences. Results: HBVDNA specific fluorescent signal was detected in the chromosomes of one patient with chronic persistent hepatitis B. 4 pairs of sperm chromosome complement, 9 pairs of specific fluorescence signals, including one of five obvious FISH points, and the remaining 2 to 4 signal points. Fluorescent signal intensity is significantly different, the signal site distribution appears to be random. Conclusion: HBV can be integrated into human sperm chromosomes and the results suggest that HBV may be transmitted vertically to the next generation