论文部分内容阅读
目的了解一起感染性腹泻暴发疫情中诺如病毒感染的分子流行特征。方法将收集到的患者粪便标本采用实时荧光RT-PCR检测诺如病毒核酸,阳性标本进行核苷酸序列测定并进行同源性分析,确定基因型。结果 2015年2月暴发的感染性腹泻疫情中,18份粪便标本中检出诺如病毒核酸阳性12份,阳性率为66.7%。对测序成功的11株诺如病毒进行序列比对,结果显示核苷酸序列同源性为100%。RdRp基因进化树分析结果显示,所测序列与2014年香港分离的诺如病毒GⅡ.17型参考株KP998539在同一个进化分支上,核苷酸序列同源性为100%。将序列提交到GenBank数据库,获得注册序列号为KP857570。结论本次疫情是由GⅡ.17型诺如病毒感染引起的感染型腹泻暴发疫情。
Objective To understand the molecular epidemiology of norovirus infection in an outbreak of infectious diarrhea. Methods The stool specimens collected from patients were detected by real-time fluorescence RT-PCR for detection of Norovirus nucleic acid. The positive samples were sequenced and their homologies were analyzed to determine the genotypes. Results Of the infectious diarrhea outbreaks in February 2015, 12 positive samples of norovirus were found in 18 stool samples, the positive rate was 66.7%. Sequencing of 11 Norovirus strains sequenced successfully showed that the nucleotide sequence homology was 100%. The phylogenetic tree analysis of RdRp revealed that the nucleotide sequence homology was 100% with that of Norovirus GⅡ.17 reference strain KP998539 isolated from Hong Kong in 2014 on the same branch. The sequence was submitted to the GenBank database, access to registration serial number KP857570. Conclusion The outbreak was caused by outbreak of infectious diarrhea caused by GⅡ.17 Norovirus infection.