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目的 探讨丙型肝炎病毒 (HCV)感染对肾移植患者免疫功能的影响。方法 提取患者外周血的淋巴细胞 ,应用碱性磷酸酶 抗碱性磷酸酶 (APAAP)酶桥联法检测CD3+、CD4+、CD8+及CD16 +细胞 ;免疫沉淀法测定血清中免疫球蛋白和补体水平。结果 HCV IgG和 /或HCVRNA阳性者 (HCV阳性组 )CD4+/CD8+值为 0 .90± 0 .2 5 ,显著低于HCV阴性组的 1.46± 0 .5 4(P <0 .0 5 ) ,主要是CD4+细胞下降显著 ;HCV阳性组CD16 +细胞高达 2 7.8% ,与HCV阴性组比较 ,差异有显著性 (P <0 .0 1)。HCV阳性组和阴性组的血清各免疫球蛋白和补体的差异无显著性 (P >0 .0 5 )。结论 HCV阳性的肾移植患者其免疫功能紊乱。
Objective To investigate the effect of hepatitis C virus (HCV) infection on immune function in renal transplant recipients. Methods Peripheral blood lymphocytes were extracted from patients. The levels of CD3 +, CD4 +, CD8 + and CD16 + were detected by alkaline phosphatase - resistant alkaline phosphatase (APAAP) enzyme - linked immunosorbent assay. The levels of immunoglobulins and complement were measured by immunoprecipitation. Results CD4 + / CD8 + values of HCV IgG and / or HCV RNA positive group (HCV positive group) were 0.90 ± 0.52, significantly lower than those of HCV negative group (1.46 ± 0.54, P <0.05) Mainly CD4 + cells decreased significantly; HCV positive group CD16 + cells up to 2 7.8%, compared with the HCV-negative group, the difference was significant (P <0.01). There was no significant difference in serum immunoglobulin and complement between HCV positive and negative groups (P> 0.05). Conclusion The immune function of HCV-positive renal transplant patients is disorder.