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通过免疫转移吸附试验，我们检出精浆内有抗血栓素Ⅲ抗原的存在，含量约为血浆的百分之一，分子量约５７ｋＤ。体外实验证明，纯抗血栓素Ⅲ能抑制人、猪和小鼠精子顶体素活性，其中抑制猪顶体素的二级速率常数为Ｍ－１ｓ－１，这种抑制反应是通过在ＳＤＳ－ＰＡＧＥ上形成稳定的复合物而起作用的。小鼠体外受精试验表明，在培养基中加入抗血栓素Ⅲ能抑制精－卵结合和受精率。本研究提示，抗血栓素Ⅲ不仅存在于血凝过程中，而且还可能是生殖过程的调节因子。
By immunoadsorption adsorption test, we detected the presence of anti-thromboxane III antigen in seminal plasma, the content is about one percent of the plasma, the molecular weight of about 57kD. In vitro experiments show that pure anti-thromboxane III can inhibit human, pig and mouse sperm acrosin activity, which inhibits Porcine apocrine second-order rate constant for the M-1s-1, this inhibition by SDS- PAGE to form a stable complex and work. In vitro fertilization tests in mice showed that adding anti-thromboxane III to the medium inhibited both sperm-egg binding and fertilization rates. This study suggests that anti-thromboxane III not only exists in the blood coagulation process, but also may be a regulator of the reproductive process.