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目的分析非γδ T细胞型的T细胞淋巴瘤患者外周血中T细胞受体γ(TCR γ)亚家族可变区Ⅰ、Ⅱ、Ⅲ(TRGVⅠ、Ⅱ、Ⅲ)基因和预后相关趋化因子及其受体基因(CCL20、CCR6、CCL17、CCL22以及CCR4)的表达情况,探讨γδ T细胞与趋化因子/受体表达的相关性及其与疾病转归的相关性。方法收集27例非γδ T细胞型的T细胞淋巴瘤患者外周血样本,同时收集9例健康志愿者外周血样本作为对照。采用荧光实时定量PCR检测T细胞淋巴瘤患者以及正常人外周血中TRGVⅠ~Ⅲ亚家族基因以及CCL20/CCR6、CCL17/CCL22/CCR4基因的表达水平。结果初发组、难治复发组和缓解组的TRGVⅠ~Ⅲ亚家族的表达水平与正常组相比均有不同程度的增高,且TRGV亚家族表达模式发生改变。初发组和难治复发组的CCL22和CCR4的表达水平显著高于正常组,而CCL17表达水平显著低于正常组。初发、缓解和难治复发组的患者外周血中TRGV各亚家族基因与各趋化因子及受体基因表达水平的相关性模式也发生改变。初发组和难治复发组存在低表达的TRGVⅡ亚家族,治疗缓解后TRGVⅡ亚家族的表达升高。结论 TRGVⅡ亚家族可能是抗T细胞淋巴瘤的T细胞功能亚群,可能与疾病的发病和转归相关。T细胞淋巴瘤中高表达水平的CCL22/CCR4,可能与疾病的发病有关。
Objective To analyze the expression of TRGVⅠ, Ⅱ, Ⅲ and TCR-related chemokines in peripheral blood of patients with non-γδ T cell type T cell lymphoma (CCL20, CCR6, CCL17, CCL22 and CCR4), and to investigate the correlation between γδ T cells and chemokine / receptor expression and their relationship with disease outcome. Methods Twenty-seven peripheral blood samples from non-γδ T-cell T-cell lymphoma patients were collected, and peripheral blood samples from 9 healthy volunteers were collected as control. Real-time quantitative PCR was used to detect the expression of TRGVⅠ-Ⅲ subfamily genes and CCL20 / CCR6 and CCL17 / CCL22 / CCR4 genes in peripheral blood of patients with T-cell lymphoma and normal controls. Results The expression levels of TRGVⅠ ~ Ⅲ subfamilies in primary group, refractory relapse group and remission group were all increased to some extent compared with normal group, and the expression pattern of TRGV subfamily changed. The expression levels of CCL22 and CCR4 in the primary group and refractory relapse group were significantly higher than those in the normal group, while the expression level of CCL17 was significantly lower than that in the normal group. The pattern of the correlation between TRGV subfamily genes and the expression levels of various chemokines and receptor genes in peripheral blood of patients with primary, remission and refractory relapse also changed. There was a low expression of TRGVⅡ subfamily in the primary and refractory relapse groups, and the expression of TRGVⅡ subfamily increased after treatment. Conclusion The TRGVⅡ subfamily may be a T cell subset of anti-T-cell lymphoma, which may be related to the onset and outcome of the disease. The high expression level of CCL22 / CCR4 in T-cell lymphoma may be related to the pathogenesis of the disease.