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目的:优选从棉子中分离棉酚的大孔吸附树脂纯化工艺。方法:采用静态吸附-洗脱与动态吸附-洗脱试验筛选大孔树脂型号,通过单因素试验优选洗脱流速,利用正交试验考察上样液质量浓度、洗脱剂乙醇体积分数、上样液pH及洗脱液中乙醇体积分数对棉酚纯化工艺的影响。结果:选用H103型大孔吸附树脂,最佳纯化工艺为上样液中棉酚质量浓度90 mg·L-1,上样液pH 7,上样液中乙醇体积分数40%,洗脱剂为80%乙醇;棉酚纯度由提取液的6.2%提高至洗脱液中83.6%,转移率76.7%。结论:优选的工艺稳定可行,H103型大孔树脂适用于棉酚的纯化。
Objective: To optimize the purification of macroporous adsorption resin from gossypol in cottonseed. Methods: The macroporous resin model was screened by static adsorption-elution and dynamic adsorption-elution test, and the elution flow rate was optimized by single factor test. The orthogonal design was used to investigate the concentration of the sample solution, the eluent ethanol volume fraction, Effect of Liquid pH and Ethanol Volume Fraction in Eluent on Purification of Gossypol. Results: The optimal purification process for the macroporous resin H103 was as follows: the concentration of gossypol in the sample solution was 90 mg · L-1, the pH of the sample solution was about 7, the volume fraction of ethanol in the sample solution was 40%, and the eluent was 80% ethanol. The purity of gossypol increased from 6.2% of the extract to 83.6% of the eluate with the transfer rate of 76.7%. Conclusion: The preferred process is stable and feasible. H103 macroporous resin is suitable for the purification of gossypol.