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溶磷细菌是主要的根际促生菌的类群之一,其在发展绿色农业和荒漠化防治中具有重要作用。已有的相关研究主要集中于农作物等,而对于荒漠草原植物溶磷细菌的研究尚很缺乏。该研究利用涂布划线方法、以无机磷培养基从四子王旗荒漠草原四种优势植物根际土壤中分离溶磷细菌,采用过硫酸钾消解-钼锑抗比色法检测菌株的溶磷能力。从4种优势植物兔唇冬青草、银灰旋花、蒙古黄芪和短花针茅根际土壤共分离得到42株溶磷细菌,归为五个类群19个属。其中19株(45.2%)属于放线菌纲(Actinobacteria),5株(11.9%)属于芽孢杆菌纲(Bacilli),16株(38.1%)属于α-变形菌纲(α-proteobacteria),1株(2.4%)属于β-变形菌纲(β-proteobacteria),1株(2.4%)属于γ-变形菌纲(γ-proteobacteria)。溶磷能力测定结果表明菌株GP0501、GP0506、GP0507和GP0509在42株菌种中溶磷能力最强,溶磷量分别为77.57、65.89、58.08、68.02μg/mL,都属于芽孢杆菌属(Bacillus),具有较大的应用潜力。
Phosphorus-dissolving bacteria are one of the major rhizosphere-promoting bacteria and play an important role in the development of green agriculture and desertification control. Existing research has mainly focused on crops, etc. However, there is still a lack of research on phosphate-solubilizing bacteria in desert steppe plants. In this study, phosphate-solubilizing bacteria were isolated from rhizosphere soil of four dominant plants in Siziwangqi desert steppe by inorganic phosphorus medium by coating-scribing method. The ability of phosphorus-solubilizing bacteria was tested by potassium persulfate digestion-molybdenum antimony colorimetry. A total of 42 phosphate-solubilizing bacteria were isolated from the rhizosphere soils of four dominant species, Lost alpine, Agrocybe aegerlus, Mongolian Astragalus and Stipa breviflora and classified as 19 genera of five taxa. Among them, 19 (45.2%) belonged to Actinobacteria, 5 (11.9%) belonged to Bacilli, 16 (38.1%) belonged to α-proteobacteria and 1 (2.4%) belonged to β-proteobacteria and 1 strain (2.4%) belonged to γ-proteobacteria. The results of the determination of the ability of dissolving phosphorus showed that the strains GP0501, GP0506, GP0507 and GP0509 had the strongest ability to dissolve phosphorus in 42 strains, the amounts of dissolved phosphorus were 77.57, 65.89, 58.08 and 68.02 μg / mL respectively, belonging to Bacillus , Has great potential applications.