作者对14例生前无耳病的死者,死后72小时内,取下颞骨,置甲醛-酒精(1:9)液固定2～4周,固定后分别将鼓膜松弛部与紧张部取出,按Smith和Atkinson氏法。将标本浸入含0.25％甲苯胺蓝及0.5％浓盐酸、70％酒精的染液中,置室温下染色15～30分钟,再将其置95％酒精内以去除多余染料,如着色 The authors of 14 deceased patients without ear disease, within 72 hours after death, remove the temporal bone, formaldehyde - alcohol (1: 9) solution for 2 to 4 weeks, respectively, after the tympanic membrane relaxation and tension were removed by Smith and Atkinson’s method. The specimen is immersed in a dye liquor containing 0.25% toluidine blue and 0.5% concentrated hydrochloric acid, 70% alcohol, stained for 15 to 30 minutes at room temperature, and then placed in 95% alcohol to remove excess dye, such as coloring