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用Ｎｏｒｔｈｅｒｎ印迹杂交对１５份人肝细胞癌（ＨＣＣ）、５例癌旁肝硬变和３例正常肝组织标本中整合素α５、β１亚基和纤维连接蛋白（ＦＮ）ｍＲＮＡ表达作杂交分析。结果：低分化ＨＣＣ癌组织中整合素α５、β１亚基和ＦＮｍＲＮＡ表达水平明显低于高分化ＨＣＣ及癌旁和正常肝组织（Ｐ＜０．０１）；ＨＣＣ伴肝内浸润和（或）转移组癌组织内３种ｍＲＮＡ水平较无浸润转移组低，差异有显著性意义（Ｐ＜０．０５）；５例伴肝内转移的ＨＣＣ癌组织ＦＮｍＲＮＡ呈异质性表达（５．０ｋｂ位置）。提示：ＦＮ及其受体整合素α５、β１基因转录水平的改变与ＨＣＣ细胞分化、浸润和转移密切相关；异质性ＦＮｍＲＮＡ及其编码的ＦＮ蛋白可能在ＨＣＣ转移中有意义。
Northern blot hybridization was used to analyze the expression of integrin α5, β1 subunit and fibronectin (FN) mRNA in 15 human hepatocellular carcinoma (HCC), 5 paracancerous liver cirrhosis and 3 normal liver tissue samples. Results: The expression levels of integrin α5, β1 subunit and FN mRNA in poorly differentiated HCC cancer tissue were significantly lower than those in well-differentiated HCC and adjacent and normal liver tissues (P<0.01); HCC was associated with intrahepatic infiltration and/or metastasis. The three mRNA levels in the group of cancer tissues were lower than those in the non-invasion and metastasis groups (P<0.05); the heterogeneity of FN mRNA expression in 5 cases of HCC cancer tissues with intrahepatic metastasis (5.0kb) . It is suggested that the transcriptional changes of FN and its receptor integrins α5 and β1 genes are closely related to the differentiation, infiltration and metastasis of HCC cells. Heterogenous FN mRNA and the encoded FN protein may be significant in the metastasis of HCC.