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目的:探讨甲醛和苯联合作用对小鼠骨髓细胞DNA的损伤作用。方法:选用90只雌性昆明小鼠为研究对象,采用2×3析因设计,随机分为9组,即阴性对照组、甲醛低浓度组(F_1:10 mg/m~3)、甲醛高浓度组(F_2:20 mg/m~3)、苯低浓度组(B_1:200 mg/m~3)、苯高浓度为(B_2:400 mg/m~3)、甲醛低+苯低组(F_1:10mg/m~3+B_1:200 mg/ m~3)、甲醛低+苯高组(F_1:10 mg/m~3+B_2:400 mg/m~3)、甲醛高+苯低组(F_2:20mg/m~3+B_2:200 mg/m~3)、甲醛高+苯高组(F_2:20 mg/m~3+B_2:400mg/m~3),对各组小鼠进行静式染毒,2 h/d,连续3个月,染毒期结束后将其全部处死,利用彗星试验检测小鼠骨髓细胞彗星细胞率(DNA受损伤的细胞的率)和彗星细胞的尾长(损伤程度)。结果:各染毒组小鼠骨髓细胞彗星细胞率、彗星细胞的尾长均高于对照组彗星细胞率和彗星细胞尾长(P<0.05),小鼠骨髓细胞的彗星细胞率和彗星细胞尾长随甲醛或苯剂量的增加而增加(P<0.05),高剂量联合组慧星细胞率和彗星细胞尾长高于其余各组(P<0.05)。结论:甲醛和苯对骨髓细胞的彗星细胞率和彗星尾长的作用高于其余各组,甲醛和苯表现为相加作用。
Objective: To investigate the combined effect of formaldehyde and benzene on DNA damage in mouse bone marrow cells. Methods: Totally 90 female Kunming mice were selected as experimental subjects. They were randomly divided into 9 groups (negative control group, low concentration of formaldehyde group (F 1: 10 mg / m 3), high concentration of formaldehyde (F_2: 20 mg / m ~ 3), benzene low concentration (B_1: 200 mg / m ~ 3), benzene high concentration (B_2: 400 mg / (10 mg / m ~ 3 + B_1: 200 mg / m ~ 3), low formaldehyde + benzene high group F_2: 20mg / m ~ 3 + B_2: 200mg / m ~ 3), high formaldehyde group + benzene high group (F_2: 20mg / m ~ 3 + B_2: 400mg / m ~ (2 h / d) for 3 consecutive months. All the mice were sacrificed after the end of the exposure period. The comet assay was used to detect the rate of comet cells (the rate of damaged DNA) and the tail length of comet cells (degree of damage). Results: The rate of comet cells and the tail length of comet cells were higher in mice than in control group (P <0.05). The rates of comet cells and comet cells in mouse bone marrow The length of comet cells increased with the increase of formaldehyde or benzene dosage (P <0.05). CONCLUSION: Formaldehyde and benzene have higher effect on the rate of comet cells and tail length of myeloid cells than the other groups, and formaldehyde and benzene show an additive effect.