汤飞凡等于1957年首次用鸡胚分离沙眼衣原体成功之后,1963年Gordon等则用照射的McCoy细胞分离沙眼衣原体获得成功。此后,一些研究者认为此法较鸡胚方法敏感、经济、快速和较易纯化,故国外广泛应用。Evans等比较了6种不同处理方法对McCoy After Tang Feifan was equal to the first successful isolation of Chlamydia trachomatis with chicken embryo in 1957, Gordon et al. Succeeded in isolating Chlamydia trachomatis from irradiated McCoy cells in 1963. Since then, some researchers believe that this method is more sensitive, more economical, faster and easier to purify than chicken embryo, so it is widely used abroad. Evans et al. Compared six different treatments to McCoy