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从稗叶枯病株分离病原菌(Helminthosporium monoceras D rechsler)11个菌株,其培养滤液均可抑制稗的胚根生长,与对照比胚根平均相对生长率为28.47%～53.60%,适于菌株的生长和毒素产生的培养液为10%稗汁葡萄糖和改良Fires培养液,适于菌株生长和产毒的温度为25～30℃,pH 6～7.通气有利于菌株的生长,但对产毒无显著影响,连续光照或连续黑暗有利于菌株产毒,菌株在pH 6～7的PDA培养基上,于30℃连续黑暗的培养产孢量最多。
Eleven strains of Helminthosporium monoceras D rechsler were isolated from the leaves of barnyardgrass blight. The culture filtrate could inhibit the growth of barnyardgrass. Compared with the control, the average relative growth rate of radicle was 28.47% ~ 53.60% Growth and toxin production medium is 10% barnyard grass glucose and modified Fires culture medium, suitable for the growth of strains and toxin production temperature 25 ~ 30 ℃, pH 6 ~ 7. Ventilation is conducive to the growth of strains, but the toxic No significant effect, continuous light or continuous dark is conducive to the production of strains, strains in pH 6 ~ 7 PDA medium, 30 ℃ continuous dark culture sporulation most.