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目的:探讨中期因子(MK)蛋白在非小细胞肺癌(NSCLC)中的表达水平、特点及其与NSCLC血管生成和预后的关系。方法:采用免疫组织化学方法检测44例NSCLC组织中MK蛋白表达和微血管密度(MVD),并与临床病理及预后指标作对照分析。结果:在癌旁及周围正常组织中,MK无表达;而在NSCLC癌细胞胞质中存在着MK的高表达,其表达阳性率为59.1%。MK表达与微血管密度增高、淋巴结转移显著相关(P<0.01),术后生存时间较MK阴性患者显著缩短(P<0.05)。MVD水平升高与淋巴结转移显著相关(P<0.01),且患者生存期显著缩短(P<0.05)。结论:MK蛋白在NSCLC中过表达,与肿瘤侵袭及血管生成有关,其表达水平能反映NSCLC的恶性程度,有可能作为NSCLC转移和预后分析的指标。
Objective: To investigate the expression of midkine (MK) protein in non-small cell lung cancer (NSCLC) and its relationship with angiogenesis and prognosis in NSCLC. Methods: Immunohistochemistry was used to detect MK protein expression and microvessel density (MVD) in 44 NSCLC tissues, and compared with clinicopathological parameters and prognostic factors. Results: There was no expression of MK in the normal and adjacent tissues. The high expression of MK was found in the cytoplasm of NSCLC, and the positive rate of MK was 59.1%. MK expression was significantly associated with increased microvessel density and lymph node metastasis (P <0.01), and postoperative survival time was significantly shorter than that of MK negative patients (P <0.05). The level of MVD was significantly associated with lymph node metastasis (P <0.01), and the survival time was significantly shorter (P <0.05). Conclusion: MK protein is overexpressed in NSCLC, which is related to tumor invasion and angiogenesis. The expression level of MK protein reflects the malignancy of NSCLC and may be used as an indicator of metastasis and prognosis of NSCLC.