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目的为了解新生儿期测定一氧化氮（ＮＯ）时的影响因素，研究脐血溶血是否左右ＮＯ的检测结果。方法分娩时收集母亲健康、生产史无异常的正常新生儿脐血３４份，每份样本同时分装两管，并使其中１管溶血，以１５００ｒ／ｍｉｎ的速度离心１０ｍｉｎ分别分离血清，应用硝酸还原酶法测定３４例样本的溶血血清及与其相匹配的３４例非溶血血清ＮＯ的浓度。结果溶血标本ＮＯ浓度为（１３．７２±１１．９２）μｍｏｌ／Ｌ，明显低于非溶血标本（５３．９５±２６．２７）μｍｏｌ／Ｌ（ｔ＝７．１９３，Ｐ＜０．００１）。结论提示脐血溶血可明显降低ＮＯ的检出量。
Objective To understand the influencing factors in the determination of nitric oxide (NO) during the neonatal period and to investigate whether oxytocin is associated with NO. Methods The mother’s health was collected during delivery. 34 normal neonatal umbilical blood samples were collected. Each sample was divided into two tubes at the same time. One tube was hemolyzed and centrifuged at 1500r / min for 10 min to separate the serum. Nitric acid 34 cases of hemolytic sera and 34 cases of non-hemolytic serum NO concentrations measured by reductase method. Results The concentration of NO in hemolysis samples was (13.72 ± 11.92) μmol / L, which was significantly lower than that in non-hemolysis samples (53.95 ± 26.27 μmol / L, t = 7.193, . Conclusions Umbilical cord blood hemolysis can significantly reduce the detection of NO.