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目的 :研究两种新型杀虫剂 -吡虫啉和抑食肼对人体外周血淋巴细胞遗传物质的影响。方法 :人体外周血淋巴细胞的微核试验 ,姐妹染色单体互换试验 (SCE)以及单细胞凝胶电泳试验 (SCGE ,又名彗星试验 )。结果 :在低浓度 (吡虫啉为 0 .0 5mg/L ,抑食肼为 5mg/L)时 ,它们对微核和SCE的影响与对照组相比无显著性差异 (P >0 .0 5 ) ,当浓度升高 (吡虫啉为 0 .1mg/L ,抑食肼为 2 5mg/L)时 ,则有显著性差异 (P <0 .0 5 )。而彗星试验在各试验组与对照组相比都有极显著性差异 (P <0 .0 1) ,且存在明显的剂量 -效应关系 (r =0 .995 ,r=0 .96 5 )。结论 :吡虫啉和抑食肼对人外周血淋巴细胞的遗传物质都具有一定程度的损伤作用 ,相比之下 ,吡虫啉比抑食肼具有更大的毒性。
OBJECTIVE: To study the effects of two new insecticides, imidacloprid and forbidden hydrazine, on the genetic material of human peripheral blood lymphocytes. Methods: Human peripheral blood lymphocyte micronucleus test, sister chromatid exchange test (SCE) and single cell gel electrophoresis test (SCGE, also known as comet assay). Results: There was no significant difference in micronucleus and SCE between imidacloprid and SCE at low concentration (0,05 mg / L imidacloprid and 5 mg / L for hydrolysable hydrazine) (P> 0.05) There was a significant difference (P <0.05) when the concentration was increased (imidacloprid was 0.1 mg / L, and the inhibition of hydrazine was 25 mg / L). The comet assay showed significant difference (P <0.01) in all experimental groups compared with the control group, and there was a significant dose-response relationship (r = 0.995, r = 0.96 5). CONCLUSION: Imidacloprid and anti-inducible hydrazine exert a certain degree of damage on the genetic material of human peripheral blood lymphocytes. In contrast, imidacloprid is more toxic than hydrazine-alone.