Correlation of Lower Concentrations of Hydrogen Sulfide with Activation of Protein Kinase CβⅡ in Ure

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Background:Hydrogen sulfide (H2S) plays a protective role in chronic hemodialysis (CHD) patients.In this study,we further investigate the relationship between H2S and conventional protein kinase CβⅡ (cPKCβⅡ) in CHD patients with uremic accelerated atherosclerosis (UAAS).Methods:A total of 30 healthy people,30 CHD patients without AS and 30 CHD patients with AS (CHD + AS) were studied.Plasma H2S was measured with a sulfide sensitive electrode,and cPKCβⅡ membrane translocation was detected by West blotting.Results:Plasma H2S in CHD + AS group was significantly lower than that in CHD patients.cPKCβⅡ membrane translocation in CHD + AS group increased significantly compared with CHD group.Plasma H2S concentration was negatively correlated with cPKCβⅡ membrane translocation in CHD + AS patients.Conclusions:These findings suggest a possible linkage between H2S metabolism and cPKCβⅡ activation,which may contribute to the development of UAAS in CHD patients.
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