论文部分内容阅读
掌叶半夏(Pinellia pedatisecta Schott)种子在附加2,4-D2.0,BA0.5mg/L的MS培养基上形成浅黄色或白色颗粒状胚性愈伤组织。胚性愈伤组织在附加2,4-D1.0,BA0.5,CH300mg/L的MS液体培养基中振荡培养,可产生大量的体细胞胚。2,4-D对体胚诱导效果显著并促进其早期发育,但抑制其进一步发育成熟。NAA对体胚诱导效果不如2,4-D,但可使体胚正常发育。水解酪蛋白明显提高体胚诱导频率。显微观察表明:体胚起源于单个胚性细胞,胚性单细胞经极性分裂形成二细胞、三细胞、多细胞原胚,再经球形胚、梨形胚、子叶分化期胚到成熟胚;同时,还观察到类似双子叶植物合子胚发育过程中的心形胚和鱼雷胚。另外,还观察到连体胚、次生胚等畸形胚。成熟体胚转移到分化培养基上,可发育为正常小植株。
Pinellia pedatisecta Schott seeds formed light yellow or white granular embryogenic callus on MS medium supplemented with 2,4-D2.0 and BA0.5mg / L. Embryogenic callus cultured in MS liquid medium supplemented with 2,4-D1.0, BA0.5, CH300mg / L, can produce a large number of somatic embryos. 2,4-D on the somatic embryo induction significantly and promote its early development, but inhibit its further development and maturation. NAA is less effective than somatic embryos in inducing 2,4-D, but allows normal somatic embryogenesis. Hydrolyzed casein significantly increased somatic embryo induction frequency. Microscopic observation showed that the somatic embryos originated from a single embryogenic cell. The embryogenic single cells were divided into two cells, three cells and multicellular proembryos by polar division. At the same time, heart-shaped embryos and torpedo-like embryos were also observed during the development of zygotic zygotic embryos. In addition, deformed embryos such as Siamese embryos and secondary embryos were also observed. Mature somatic embryos transferred to differentiation medium can develop into normal plantlets.