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目的采用RP-HPLC测定麝香草酚的含量和有关物质。方法用C18色谱柱,流动相为水-甲醇(40∶60),流速为1.0 mL.min-1,检测波长276 nm。结果麝香草酚含量测定的线性范围为0.5014～2.0055 mg.mL-1(r=0.9999),RSD=0.20%;有关物质的线性范围为50.1～501.4μg.mL-1(r=0.9999);最低检测限为30 ng;最小定量限为98 ng。结论所用方法操作简便,结果准确可靠,可用于麝香草酚的含量测定和有关物质检查。
Objective To determine the content of thymol and related substances by RP-HPLC. Methods The chromatographic column was C18, the mobile phase was water-methanol (40:60), the flow rate was 1.0 mL.min-1 and the detection wavelength was 276 nm. Results The linear range of thymol content was 0.5014 ~ 2.0055 mg.mL-1 (r = 0.9999) with RSD 0.20%. The linear range of the related substances was 50.1 ~ 501.4μg.mL-1 (r = 0.9999) The limit of detection is 30 ng; the limit of quantification is 98 ng. Conclusion The method is simple, accurate and reliable, and can be used for the determination of thymol and related substances.