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近年来,不少作者认为大肠癌与乳腺癌一样,为雌激素依赖性肿瘤,并进一步探索这些激素依赖性肿瘤的共同病因及抗雌激素治疗的临床效果。1986年1月至1989年1月我们对68例大肠癌(直肠癌49例、左半结肠癌10例,右半结肠癌9例)作了雌激素受体(ER)及孕酮受体(PgR)测定,并就其与肿瘤部位及组织学类型的关系作了研究。1 材料与方法1.1 标准来源测定ER及PgR的材料全部来源于本院手术切除标本,诊断均经病理证实。1.2 测定方法测定ER及PgR均采用荧光组织化学法,以17β-Estradiol-BSA-FITC测定雌激素受体,Progesterone-BSA-TMRITC测定孕酮受体。将大肠癌或转移淋巴结的新鲜标本作6～8μ厚度的冰冻切片,用10~(-4)克分子浓度的非荧光雌二醇作竞争性抑制试验,并将另一对照标本预先加热 70℃,30分钟,作加温试验。如用荧光激素孵育的标本,
In recent years, many authors believe that colorectal cancer, like breast cancer, is an estrogen-dependent tumor, and further explore the common causes of these hormone-dependent tumors and the clinical effects of anti-estrogen therapy. From January 1986 to January 1989, we performed estrogen receptor (ER) and progesterone receptors in 68 cases of colorectal cancer (49 cases of rectal cancer, 10 cases of left colon cancer, and 9 cases of right colon cancer). PgR) was measured and studied in relation to tumor site and histological type. 1 Materials and methods 1.1 Standard sources The materials for the determination of ER and PgR are all derived from surgical specimens from this hospital. The diagnosis was confirmed by pathology. 1.2 Determination of the determination of ER and PgR using fluorescence histochemistry, 17β-Estradiol-BSA-FITC determination of estrogen receptors, Progesterone-BSA-TMRITC determination of progesterone receptors. Fresh specimens of colorectal cancer or metastatic lymph nodes were frozen at a thickness of 6 to 8 μm, and a non-fluorescing estradiol concentration of 10~(-4) was used as a competitive inhibition test, and another control sample was pre-heated at 70°C. , 30 minutes for heating test. For specimens incubated with fluorescent hormones,